G. N. Singina
L.K. Ernst Federal Research Center for Animal Husbandry, pos. Dubrovitsy, 60, Podol’sk, Moskovskaya obl., 142132, Russian Federation
Abstract. Technology of in vitro embryo production (IVP) and its implementation into the practice of cattle farming can increase the rate of genetic progress in breeding and ensure the conservation of genetic biodiversity in this species. Efficiency of IVP technology can be increased by optimizing the in vitro embryo culture conditions (IVC). This paper was the first to compare two IVC protocols that involve culture of embryos in commercial medium BO-IVC either under 5 % СО2, 5 % О2 и 90 % N2 (group 1) or under 6 % СО2, 5 % О2 и 89 % N2 in air (group 2). Slaughterhouse-derived bovine oocytes (n=222) were cultured in the maturation medium, in vitro fertilized with the frozen-thawed sperm and transferred in the culture medium for embryo development. After 7 days of culture the number of embryos developed to the blastocyst stage (blastocyst rate) and their quality were evaluated, and the rate of embryos arrested before16 cell stage was determined. The rate of embryos with less than 16 cells in group 1 was 39,17 ± 4,89 %. Increasing the CO2 concentration in air to 6 % (group 2) significantly reduced this rate (p < 0.05). In addition, the effect of the gas atmosphere composition on the development of embryos to the blastocyst stage was found. When embryos cultured in BO-IVC medium under 5 % СО2, 5 % О2 и 90 % N2 in air the blastocyst rate was 31,60 ± 3,04 %. Using for culture of 6 % СО2, 5 % О2 и 90 % N2 increased this rate to 42,30 ± 3,20 % (p < 0,05). Nevertheless, total number of nuclear in blastocyst was similar in both groups and averaged 90 cells. Thus, BO-IVC commercial medium provides high efficiency of IVP technology production in cattle embryos. Also, it is obvious that in the case of culture of embryos in this medium, the incubator gas composition containing 6 % CO2, 5 % O2 and 90 % N2 is preferable to the traditional one containing 5 % CO2.
Keywords: cattle, in vitro, embryos, culture medium.
Author Details: G. N. Singina, Cand. Sc. (Biol.), head of laboratory (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).
For citation: Singina GN. [Optimization of gas atmosphere parameters for culture of bovine embryos in vitro] Dostizheniya nauki i tekhniki APK. 2022;36(10):72-77. Russian. doi: 10.53859/02352451_2022_36_10_72.